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. 2014 Feb 18;5(1):24. doi: 10.1186/scrt413

Table 2.

Summary of proteasome inhibitors and their impact on pluripotency

Cell type Concentration, inhibitor/time Effect Pluripotency marker Reference
HFF/iPSC-derived fibroblast
250, 500 and 1,000 nM MG132/40 hours
No morphological change
Nondetectable
[21]
Fibroblasts
2 μM UK101/PD957
Complete inhibition of reprogramming
Nondetectable
[40]
MEFs
Low doses of MG132
Complete inhibition of reprogramming
Nondetectable
[32]
hESC-derived fibroblasts
250, 500 and 1,000 nM MG132/40 hours
No morphological change
Nondetectable
[21]
hESCs
125 nM MG132/40 hours
No effect
Significant downregulation of OCT4, SOX2, NANOG and TRA-1-60
[21]
 
250 nM MG132/40 hours
Large patches of differentiated areas
 
 
 
500 nM MG132/40 hours
Only differentiated cells
 
 
 
1,000 nM MG132/40 hours
Detachment of undifferentiated cells
 
 
hESCs
62.5 nM MG132/24 hours
Downregulation of pluripotent markers and modified levels of specific germ-layer markers (upregulation of FGF5 and GATA4)
Significant downregulation of OCT4, NANOG, SOX2 and ZFP42
[34]
hESCs 2 μM UK101/PD957 for 4 days Pluripotency diminished, less alkaline phosphatase positive colonies, upregulation of FGF5 and GATA4 Significant downregulation of TRA-1-81 and SSEA3 [40]

Application of distinct proteasome inhibitors on different cell types, the effect of inhibition and the influence on pluripotency-associated transcription factors. hESC, human embryonic stem cell; HFF, human neonatal foreskin fibroblast; iPSC, induced pluripotent stem cell; MEF, murine embryonic fibroblast.