Table 1.
Strains and plasmid used in this study
| Strains and plasmids | Relevant features | Reference |
|---|---|---|
|
Escherichia coli strain |
|
|
| NovaBlue |
endA1 hsdR17(r K12-m K12+) supE44 thi-I gyrA96 relA1 lac recA1/F’[proAB + lacIq ZΔM15::Tn10(Tetr)] |
Novagen |
|
Aspergillus oryzae strains |
|
|
| RIB40 |
Wild type |
Machida et al.
[11] |
| NSPlD1 |
niaD
-
sC
-
adeA
-
ΔargB::adeA
-
ΔligD::argB ΔpyrG::adeA |
Maruyama and Kitamoto
[12] |
| NSPlD1/pIS1-kojR |
niaD
-
::pIS1-kojR [P-sodM::kojR::T-glaB] sC
-
adeA- ΔargB::adeA- ΔligD::argB ΔpyrG::adeA |
This study |
| NSPlD1/pIS1-kojR/pIS1sC-CBHI-EGI-BGLI |
niaD
-
::pIS1-kojR [P-sodM::kojR::T-glaB] sC- ::pIS1sC-CBHI-EGI-BGLI [P-svaA::CBHI::T-svaA, P-hlyA::EGI::T-hlyA, P-sodM::CutL-BGLI::T-glaB] adeA- ΔargB::adeA- ΔligD::argB ΔpyrG::adeA |
This study |
| Plasmids |
|
|
| pISI-CBHI |
CBHI gene from T. reesei containing plasmid |
This study |
| pIS1-svaA-CBHI |
Vector for expression of CBHI from T. reesei [P-svaA::CBHI::T-glaB]; niaD marker |
This study |
| pISI-EG |
EGI (T. reesei) gene containing plasmid |
This study |
| pIS1-hlyA-EGI |
Vector for expression of EGI from T. reesei [P-hlyA::EG::T-hlyA]; niaD marker |
This study |
| pIS1-BGLI |
Vector for expression BGLI from A. aculeatus [P-sodM::CutL-BGL::T-glaB]; niaD marker |
This study |
| pIS1-CBHI-EGI-BGLI |
Vector for expression of CBHI, EGI, and BGLI [P-svaA::CBHI::T-svaA, P-hlyA::EGI::T-hlyA, P-sodM::CutL-BGLI::T-glaB]; niaD marker |
This study |
| pIS1sC-CBHI-EGI-BGLI |
Vector for expression CBHI, EGI, and BGLI [P-svaA::CBHI::T-svaA, P-hlyA::EGI::T-hlyA, P-sodM::CutL-BGLI::T-glaB]; sC marker |
This study |
| pIS1-kojR | Vector for expression of transcription factor (AO090113000137) [P-sodM::kojR::T-glaB]; niaD marker | This study |