Fig. 2.

Cerebellar VZ progenitors produce glutamatergic neurons of DCN and UBCs in the absence of Ptf1a function. (A, C, G, and H) Sagittal sections of e15.0 cerebellar anlage regions illustrated in schematics stained with indicated antibodies. Higher magnification images (B, D, d–d’’, and h–h’’) show regions boxed in adjacent panels. (A–D, d–d’’, G, H, and h–h’’) In Ptf1a^Cre/Cre;Tau–nLacZ (Ptf1a^−/−) but not control embryos, some β-gal+ cells expressed Tbr1 (D and d–d’’, arrowheads) and Tbr2 (H and h–h’’, arrowheads). β-gal+/Tbr1+ and β-gal+/Tbr2+ double-positive cells are yellow in D and H and whitish in d’’ and h’’. (E and I) Cell counts revealed that only ∼5% of β-gal+ cells expressed Tbr1 (E) and <1% of β-gal+ cells expressed Tbr2 (I) in Ptf1a^−/− embryos (n = 4 embryos). (F and J) Quantification of Tbr1+ cells (F) and Tbr2+ cells (J) per sagittal section revealed no statistically significant difference (NS, nonsignificant; P = 0.27 for Tbr1+ cells, n = 6 embryos of each genotype, and P = 0.7 for Tbr2+ cells, n = 4 embryos of each genotype) between Ptf1a^Cre/+;Tau–nLacZ and Ptf1a^Cre/Cre;Tau–nLacZ (Ptf1a^−/−) embryos. [Scale bar, 200 µm (A and C), 95 µm (B, D, G, and H), 60 µm (d–d’’), and 50 µm (h–h’’).]