Table 2.

Thermodynamic parameters of DNA binding to the NolR Q56A mutant

DNA	n	Kd, μM	ΔG, kcal⋅mol−1	ΔH, kcal⋅mol−1	−TΔS, kcal⋅mol−1
AT	1.00 ± 0.01	0.26 ± 0.01	−8.99 ± 0.46	−3.33 ± 0.01	−5.66
AA	1.03 ± 0.01	0.82 ± 0.11	−8.30 ± 1.09	−3.77 ± 0.07	−4.53

Titrations were performed using 22-bp DNA duplexes.