Reduced water use of nhx1 nhx2 mutants. (A) Transpiration measurements of Col-0, L14, and KO plants during 4 d of drought stress. Pots were weighed twice daily, at the start of the dark period (marked as “D”) and at the onset of the light period (marked as “L”), and transpiration was calculated as the amount of water loss per area unit in each time interval (16 h dark/8 h light). Data represent the mean and SE of at least seven plants in individual pots per genotype. (B) Percentage of water loss along the drought assay in pots with Col-0, L14, and KO plants. Data represent mean and SE of at least seven plants per genotype. To quantify the background water evaporation from the soil, identical pots without plants were used as control.