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Anterior foregut endoderm specification. Representative immunofluorescent images of day 9 of differentiation indicating the colocalization of FOXA2, NKx2.1, and EpCAM (A) or PAX8 (B). (C) The same time point stained with CK18, NKx2.1, and SOX2. (D) The % of the DAPI cells stained with SOX2, NKx2.1, or both and (E) the % of the DAPI cells stained with FOXA2 or FOXA2 and NKx2.1 at day 9 of differentiation (mean ± SEM). n = 7 independent experiments. (Scale bar: 100 μm.)
