Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 7;111(17):6299–6304. doi: 10.1073/pnas.1323277111

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Freely available online through the PNAS open access option.

PMC Copyright notice

Fig. 4. — Simultaneous off-line TR-MIMS measurements of O₂ and CO₂ production by PSII. Dark-adapted PSII membranes (1 mg Chl/mL) were illuminated inside of a gas tide syringe with 100 xenon flashes and then injected into the MIMS cell either as fast as possible with our current set up (<3 s, solid lines) or after 1 min (dotted line). Displayed are the light-minus-dark difference signals of O₂ (blue traces) and CO₂ (green traces), normalized to the amplitude of O₂ release (for original traces see Fig. S3). The amplitudes of the traces for CO₂ and O₂ can be directly compared, because our set-up detects both gases with nearly equal sensitivity. Conditions: (A) 1 mM MES, pH 6.3; (B) 1 mM MES, pH 5.5; (C and D) 1 mM MES (solid lines) or 10 mM MES (dashed lines), pH 6.3. In addition to MES, the medium contained 15 mM NaCl, 15% , and 2 mM K₃[Fe(CN)₆] as electron acceptor. Zero levels are off set for clarity of presentation. In all panels the average of two to three repeats is presented.

Inline graphic — Simultaneous off-line TR-MIMS measurements of O₂ and CO₂ production by PSII. Dark-adapted PSII membranes (1 mg Chl/mL) were illuminated inside of a gas tide syringe with 100 xenon flashes and then injected into the MIMS cell either as fast as possible with our current set up (<3 s, solid lines) or after 1 min (dotted line). Displayed are the light-minus-dark difference signals of O₂ (blue traces) and CO₂ (green traces), normalized to the amplitude of O₂ release (for original traces see Fig. S3). The amplitudes of the traces for CO₂ and O₂ can be directly compared, because our set-up detects both gases with nearly equal sensitivity. Conditions: (A) 1 mM MES, pH 6.3; (B) 1 mM MES, pH 5.5; (C and D) 1 mM MES (solid lines) or 10 mM MES (dashed lines), pH 6.3. In addition to MES, the medium contained 15 mM NaCl, 15% , and 2 mM K₃[Fe(CN)₆] as electron acceptor. Zero levels are off set for clarity of presentation. In all panels the average of two to three repeats is presented.