Skip to main content
. 2014 Apr 14;111(17):6203–6208. doi: 10.1073/pnas.1400102111

Fig. 2.

Fig. 2.

[2Fe-2S]2+ cluster transfer between hGRX5 and hISCA1 followed by UV/visible spectroscopy. UV/visible spectra of holo hGRX5 (A) and His-tagged apo hISCA1 (B) were recorded before (red) and after (blue) incubation in a ∼1:1 protein ratio and their separation through nickel-affinity chromatography. (C) Reverse titration between apo hGRX5 (dashed lines) and His-tagged, holo hISCA1 (solid lines) before (red) and after (blue) their incubation and separation.