Fig. 4.

CQ transport activity of T76X mutants of PfCRT^Dd2 in Xenopus oocytes. The threonine at position 76 of PfCRT^Dd2 was replaced by each of the other 19 standard α-amino acids, and the resulting T76X mutants were tested for the ability to transport [³H]CQ into oocytes. Uptake is shown as the mean ± SEM from 3–10 separate experiments (in each experiment, measurements were made from 10 oocytes per treatment). ns, no significant difference (P > 0.05) in CQ accumulation between oocytes expressing a T76X mutant and the control (noninjected and PfCRT^HB3-expressing) oocytes. See Figs. S4C, S5C, and S6C for additional data.