NO effects on biomarker expression in MDA-MB-231 cells treated with IFN-γ (4 h) and SW (48 h). (A–E) mRNA expression of S100A8, IL-6, IL-8, and TLR4 induced by IFN-γ. (F) TIMP1 protein expression (Upper) and nitration (3NT blot) of TIMP1 immuno-precipitates (Lower) in IFN-γ–stimulated cells treated with or without AG (±SEM, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001).