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. 2012 Feb 4;27(1):54–60. doi: 10.1264/jsme2.ME11256

Table 2.

Degradation of various alkanes and alkenes by ethylene-grown ETY-M and ETY-NAG

Substrates Residual gas hydrocarbons (%)

ETY-M (A) ETY-M (B) ETY-NAG
Methane 121 (0.11) ND 98 (0.01)
Ethane 0.38 (0.05) 0.48 (0.07) 0.06 (1 × 10−4)
Ethylene ND 0.02 (0.03) 0.15 (1.3 × 10−4)
Propane 58 (0.1) 9.3 (0.03) 26 (0.02)
Propylene ND 1.0 (0.008) 4.6 (0.04)
i-Butane 122 (0.06) 91 (0.05) 102 (0.01)
n-Butane 118 (0.09) 90 (0.05) 102 (0.02)

For ETY-M, experiments ETY-M (A) and ETY-M (B) were done simultaneously but separately; 5 μL of mixed gas (A) containing 1% each of methane, ethane, propane, i-butane, and n-butane was injected into a vial for ETY-M (A), and 5 μL of mixed gas (B) containing 1% each of ethane, ethylene, propane, propylene, i-butane, and n-butane was injected into a vial for ETY-M (B). For ETY-NAG, 5 μL of mixed gas (A) and (B) was injected into a vial. Total protein concentrations of ETY-M and ETY-NAG, were 0.52 mg L−1 and 0.07 mg L−1, respectively. ND, not determined. Values given in parentheses are standard deviations for three replicates in ETY-M (A) and ETY-M (B) and are the half differences of two replicates in ETY-NAG.