Fig. 5.

Effect of efflux pump inhibitor (EPI) to viability of Exiguobacterium sp. SBH81 in the presence of acetonitrile vapor. Non-adapted cells (A) and 30-cycle acetonitrile-adapted cells (B) were grown in MSB medium supplemented with acetonitrile vapor without EPI (*) and with EPI: PAβN (0.1 mM) (■), paroxitine (0.1 mM) (●), and orthovanadate (10 mM) (▲). The initial cell OD₆₀₀ at approximately 0.19 and that at the end point at approximately 0.11 corresponded to the value ranging from 10×10⁶ and 5×10⁶ CFU mL⁻¹, respectively. Effect of EPI on cell growth in MSBYG medium in the absence of acetonitrile is shown in the inset. Data are the means of the results of at least six individual experiments.