Table 1.
Organic solvent tolerance of Exiguobacterium sp. SBH81
| Organic solvents | Log Powa | Cell survival | |
|---|---|---|---|
|
| |||
| Organic solvent at 5% (v/v) | Organic solvent at 10% (v/v) | ||
| Decane | 5.01 | +++ | +++ |
| Heptane | 4.66 | +++ | +++ |
| Octane | 5.18 | +++ | +++ |
| Decanol | 4.23 | + | + |
| Hexane | 3.90 | + | + |
| Cyclohexane | 3.44 | + | + |
| Xylene | 3.20 | − | ND |
| Ethylbenzene | 3.15 | − | ND |
| Styrene | 2.95 | − | ND |
| Toluene | 2.73 | − | ND |
| Heptanol | 2.62 | − | ND |
| Diethylphathalate | 2.42 | +++ | +++ |
| Benzene | 2.13 | ++ | ++ |
| Chloroform | 2.00 | − | ND |
| Butyl acetate | 1.78 | − | ND |
| Pentanol | 1.51 | − | ND |
| Diethyl ether | 0.89 | +++ | ND |
| Butyraldehyde | 0.88 | − | ND |
| Butanol | 0.88 | ++ | − |
| Ethyl acetate | 0.73 | − | ND |
| Propionaldehyde | 0.59 | + | ND |
| Propanol | 0.25 | ++++ | +++ |
| Ethanol | −0.31 | +++ | +++ |
| Acetonitrile | −0.34 | +++ | +++ |
| Methanol | −0.77 | +++++ | +++++ |
| Dimethylformamide | −0.87 | +++++ | ++++ |
| Dimethyl sulfoxide | −1.35 | +++++ | +++++ |
Cells were initially grown to the mid-exponential phase in MSBYG medium before organic solvent was directly added. Cell survival was examined after 12 h of solvent exposure. The number of surviving cells is represented by symbols: − (no survival) and + (survival). The number of plus signs corresponds to cell numbers (CFU mL−1): + (<102), ++ (102–104), +++ (104 to 106), ++++ (1×108–50×108), +++++ (50×108–100×108), and ND (not determined).
a
Log Pow values were according to Kowwin version 1.67, EPI Suite (US Environmental Protection Agency).