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. 2011 Nov 10;27(1):19–29. doi: 10.1264/jsme2.ME11207

Fig. 4.

Fig. 4

Neighbor-joining tree based on the alignment of approx. 750 bp 16S rRNA gene sequences of non-proteobacterial clones. Abbreviations for sampled year (deposit age, y) of clones: OY04, 2004 (3.5); OY05, 2005 (5.0); OY07, 2007 (6.6). After the representative clone, the number of similar sequences (based on a 1% cutoff) is given in parentheses. Bootstrap values from 50% to 75% and >75% are indicated by open and solid circles at the branches, respectively. I, II, and III indicate the Leptospirillum group I, II, and III, respectively. Scale bar shows 0.02 substitutions per site. Aquifex pyrophilus Kol 5aT was used as an out-group for the dendrogram.