Table 1.
Bacterial growth and production of typical secondary metabolites by five Streptomyces species grown for 4 d in MM in the absence or presence of suberin
| Dry mycelial weight (mg±SD) | Metabolite production (μg mg DW−1±SD)a | |||
|---|---|---|---|---|
|
|
|
|||
| control | + suberinb | control | + suberinb | |
| S. scabiei | 28±4 | 90±3*** | n.d. | 3.61±0.14*** |
| S. acidiscabies | 60±3 | 88±2*** | 0.05±0.00 | 1.44±0.42** |
| S. avermitilis | 27±4 | 134±3*** | n.d. | n.d. |
| S. coelicolor | 38±1 | 64±4*** | 0.35±0.20 | 1.22±0.45* |
| S. melanosporofaciens | 41±4 | 69±6** | n.d. | 1.32±0.79*** |
Values are the means of three replicates.
a
Metabolites assayed were thaxtomin A for S. scabiei and S. acidiscabies, abamectin for S. avermitilis, γ-Actinorhodin for S. coelicolor and geldanamycin for S. melanosporofaciens.
b
Values from suberin medium are significantly different from control at *: P<0.05, **: P<0.01 and ***: P<0.001 (t-test).
n.d.: not detected; detection limits were 0.05 μg, 0.05 μg and 0.1 μg of total thaxtomin A, abamectin and geldanamycin, respectively.