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Detection of plasmid pAQU1 in the donor and a representative transconjugant using PFGE (A) and Southern hybridization with the tet(M) probe (B). Lanes: M, DNA size standard (lambda ladder); 1, representative transconjugant TJ311W2; 2, E. coli W3110; 3, P. damselae subsp. damselae 04Ya311; and 4, negative control strain of P. damselae subsp. damselae JCM8967. About 20 ng of DNA was loaded in each lane.
