Table 3.
Enzyme activities of cellulolytic bacteria isolated from SDC and CRC
| Genus | Isolate No. | Hydrolysis index | ||||
|---|---|---|---|---|---|---|
|
| ||||||
| cellulase | xylanase | β-glucanase | mannanase | protease | ||
| Microbispora | SDCB3 | 4.2 ± 0.7 | 15.0 ± 4.0 | 12.9 ± 5.4 | 9.3 ± 1.3 | 11.8 ± 2.5 |
| SDCB4 | 4.9 ± 0.5 | 12.1 ± 4.0 | 8.1 ± 0.7 | 8.2 ± 1.3 | 9.9 ± 1.8 | |
| SDCB5 | 5.2 ± 1.1 | 17.2 ± 7.1 | 11.6 ± 2.7 | 9.8 ± 2.2 | 7.0 ± 2.0 | |
| SDCB7 | 5.1 ± 0.9 | 14.9 ± 4.5 | 11.6 ± 5.0 | 7.5 ± 1.2 | 5.9 ± 0.7 | |
| SDCB8 | 4.0 ± 1.1 | 28.4 ± 5.9 | 14.8 ± 1.9 | 13.4 ± 2.7 | 11.3 ± 1.7 | |
| SDCB9 | 7.7 ± 2.0 | 9.4 ± 0.9 | 10.0 ± 2.6 | 10.5 ± 1.2 | 4.9 ± 2.3 | |
|
| ||||||
| Streptomyces | SDCB1 | 3.9 ± 0.4 | 9.0 ± 0.6 | 9.4 ± 3.4 | 10.6 ± 2.3 | 3.5 ± 0.2 |
| SDCB2 | 2.7 ± 0.3 | 9.8 ± 1.4 | 7.0 ± 0.8 | 7.2 ± 0.6 | 4.4 ± 0.7 | |
| SDCB6 | 3.5 ± 0.7 | 9.7 ± 1.9 | 8.1 ± 2.3 | 7.0 ± 1.5 | 4.4 ± 0.3 | |
|
| ||||||
| Paenibacillus | SDCB10 | 4.1 ± 1.5 | 5.6 ± 1.6 | 23.7 ± 4.3 | 6.8 ± 0.9 | N.D. |
| SDCB11 | 5.0 ± 1.1 | 7.8 ± 1.4 | 16.3 ± 1.4 | 7.3 ± 1.2 | N.D. | |
|
| ||||||
| Microbispora | CRCB2 | 9.1 ± 2.9 | 9.8 ± 2.9 | 11.1 ± 1.6 | N.D. | 9.3 ± 1.4 |
| CRCB3 | 3.3 ± 0.5 | 11.3 ± 1.7 | 14.0 ± 3.7 | 9.1 ± 2.2 | 5.9 ± 1.2 | |
| CRCB4 | 4.4 ± 0.4 | 8.4 ± 1.0 | 8.3 ± 4.3 | 4.6 ± 0.5 | 6.1 ± 0.7 | |
| CRCB5 | 2.7 ± 0.4 | 7.7 ± 0.6 | 10.3 ± 2.5 | 11.5 ± 4.0 | 4.9 ± 1.0 | |
| CRCB6 | 2.5 ± 0.5 | 15.9 ± 2.2 | 14.4 ± 3.4 | 8.1 ± 2.0 | 5.3 ± 1.0 | |
| CRCB7 | 2.3 ± 0.1 | 9.9 ± 0.6 | 10.1 ± 1.5 | 11.2 ± 4.0 | 9.0 ± 2.2 | |
| CRCB8 | 3.9 ± 0.8 | 14.7 ± 0.8 | 10.8 ± 2.6 | 8.3 ± 1.5 | 7.3 ± 0.9 | |
|
| ||||||
| Streptomyces | CRCB1 | 3.1 ± 0.6 | 5.8 ± 1.1 | 4.7 ± 0.3 | N.D. | 2.7 ± 0.1 |
|
| ||||||
| Cohnella | CRCB9 | 6.1 ± 0.9 | 14.4 ± 0.6 | 9.8 ± 0.9 | 14.2 ± 2.8 | N.D. |
| CRCB10 | 6.1 ± 1.2 | 17.3 ± 3.3 | 6.5 ± 0.5 | 9.2 ± 0.3 | N.D. | |
Enzyme activities expressed as the Substrate Hydrolysis Index, which was calculated as the ratio of the average diameter (d) of the blue zone around the bacterial colony to the average diameter of the bacterial colony (D), expressed in millimeters with standard deviation in five enzyme assays using AZCL-HE-cellulose (cellulase), AZCL-arabinoxylan (xylanase), AZCL-barley β-glucan (β-glucanase), AZCL-galactomannan (mannanase) and AZCL-casein (protease). N.D.: not detected.