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Epifluorescent micrographs of Legionella pneumophila microcolonies by the MC-FA method. L. pneumophila serogroup 1 (JCM7571) cells on membrane filters were cultured on BCYEα medium for the indicated time periods and subjected to staining with FITC-labeled anti-L. pneumophila antibodies. Before incubation (a), incubated for 24 hours (b), 32 hours (c), 48 hours (d). Scale bars are 50 μm.
