Skip to main content
. 2012 Nov;158(Pt 11):2732–2741. doi: 10.1099/mic.0.062174-0

Table 2. Oligonucleotides used in this study.

No. Oligonucleotide Sequence (5′–3′)*
5586 yybTanti-GSP1 CGATGAGTGAGGTTGAGGCT
5587 yybTanti-GSP2 GGAATACTTCGATCAAGTGCTGA
5588 yybTanti-GSP3 ATCAATATGATTGAAGCAACAGC
5589 yybTanti-GSP4 ACAGCGGAATTGGTGACAGA
5590 yybTanti-GSP5 AGCCGTCACTCGTCATGGA
5591 yybTanti-GSP6 CGCCTGAAGAAGCAATGGA
5592 yybTanti-GSP7 GTCACCGAAAGCAGCAATGT
5593 yybTanti-GSP8 AACCCAATGGAGAAACGAACA
5594 yybTanti-GSP9 CGCAATCCAGCTTGGACTT
5595 yybTanti-GSP10 CTGACGTTAAGCGTCGGTGT
4549 AAP GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG
5628 sigD-up-for CTGATGGAGCTCAGTCAGGT
5629 sigD-up-rev (kan) CCTATCACCTCAAATGGTTCGCTGCCTGATCTTCATAATTCAAGGA
5630 sigD-do-for (kan) CGAGCGCCTACGAGGAATTTGTATCGTCAGATCCATTCAAAGGCATT
5631 sigD-do-rev GCTTCATAGAAATGACTGACATGT
5632 flgM-up-for TTCAGCAGAAGGTATGAATATCA
5633 flgM-up-rev(spc) CGTTACGTTATTAGCGAGCCAGTCGATATGGATTAACGGATTGTGT
5634 flgM-do-for(spc) CAATAAACCCTTGCCCTCGCTACGTCATACAAAGTAGACGCAAATCA
5635 flgM-do-rev CCGCTGTCTTGTATAACCATCA
5879 flgM-up-rev(cat) CTTGATAATAAGGGTAACTATTGCCGATATGGATTAACGGATTGTGT
5880 flgM-do-for(cat) GGGTAACTAGCCTCGCCGGTCCACGTCATACAAAGTAGACGCAAATCA
5565 PyybS-for (EcoRI) GAGGAATTCACAAAGAGGTGAAACACAATG
5566 PyybS-rev (BamHI) GAGGGATCCCAATCACAGGAACATAAACGA
5598 PyybTanti-for (EcoRI) GAGGAATTCACGAGTGACGGCTTATGTGT
5619 PyybTanti-rev2 (BamHI) GAGGGATCCGTCACCGAAAGCAGCAATGT
5620 yybT-int-for (EcoRI) GAGGAATTCATCAAACGGGATGAGCGTCTCT
5621 yybT-PDmut-up-rev TCGCTGCGCCTATGGAATCCATGTCGGGGAATTTATG
5622 yybT-PDmut-do-for CATAAATTCCCCGACATGGATTCCATAGGCGCAGCGATCGGGATTTTAAAG
5623 yybT-rev (BamHI) GAAGGATCCTCATCTCTGTACGCCTCCCT
5684 yybS-int-up-for-(EcoRI) GAGGAATTCTTGATATTGTAGAAACTGTAGCGA
5685 yybS-up-rev-(FLAG) CATCCGCGGTTTATCATCATCATCTTTATAATCCATTTCTATCACTCCCCACCATGT
5246 yybT-for N-flag 1 ATGGATTATAAAGATGATGATGATAAACCGCGGATGCCAAGCTTTTATGAAAAAC
5686 yybT-int-do-rev (BamHI) GAGGGATCCATCCAATCCTTGTGTCACATCA
5298 yybT-for AGTGATAGAAATGCCAAGCT
*

Restriction sites are underlined.