Table 2. Oligonucleotides used in this study.
No. | Oligonucleotide | Sequence (5′–3′)* |
5586 | yybTanti-GSP1 | CGATGAGTGAGGTTGAGGCT |
5587 | yybTanti-GSP2 | GGAATACTTCGATCAAGTGCTGA |
5588 | yybTanti-GSP3 | ATCAATATGATTGAAGCAACAGC |
5589 | yybTanti-GSP4 | ACAGCGGAATTGGTGACAGA |
5590 | yybTanti-GSP5 | AGCCGTCACTCGTCATGGA |
5591 | yybTanti-GSP6 | CGCCTGAAGAAGCAATGGA |
5592 | yybTanti-GSP7 | GTCACCGAAAGCAGCAATGT |
5593 | yybTanti-GSP8 | AACCCAATGGAGAAACGAACA |
5594 | yybTanti-GSP9 | CGCAATCCAGCTTGGACTT |
5595 | yybTanti-GSP10 | CTGACGTTAAGCGTCGGTGT |
4549 | AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG |
5628 | sigD-up-for | CTGATGGAGCTCAGTCAGGT |
5629 | sigD-up-rev (kan) | CCTATCACCTCAAATGGTTCGCTGCCTGATCTTCATAATTCAAGGA |
5630 | sigD-do-for (kan) | CGAGCGCCTACGAGGAATTTGTATCGTCAGATCCATTCAAAGGCATT |
5631 | sigD-do-rev | GCTTCATAGAAATGACTGACATGT |
5632 | flgM-up-for | TTCAGCAGAAGGTATGAATATCA |
5633 | flgM-up-rev(spc) | CGTTACGTTATTAGCGAGCCAGTCGATATGGATTAACGGATTGTGT |
5634 | flgM-do-for(spc) | CAATAAACCCTTGCCCTCGCTACGTCATACAAAGTAGACGCAAATCA |
5635 | flgM-do-rev | CCGCTGTCTTGTATAACCATCA |
5879 | flgM-up-rev(cat) | CTTGATAATAAGGGTAACTATTGCCGATATGGATTAACGGATTGTGT |
5880 | flgM-do-for(cat) | GGGTAACTAGCCTCGCCGGTCCACGTCATACAAAGTAGACGCAAATCA |
5565 | PyybS-for (EcoRI) | GAGGAATTCACAAAGAGGTGAAACACAATG |
5566 | PyybS-rev (BamHI) | GAGGGATCCCAATCACAGGAACATAAACGA |
5598 | PyybTanti-for (EcoRI) | GAGGAATTCACGAGTGACGGCTTATGTGT |
5619 | PyybTanti-rev2 (BamHI) | GAGGGATCCGTCACCGAAAGCAGCAATGT |
5620 | yybT-int-for (EcoRI) | GAGGAATTCATCAAACGGGATGAGCGTCTCT |
5621 | yybT-PDmut-up-rev | TCGCTGCGCCTATGGAATCCATGTCGGGGAATTTATG |
5622 | yybT-PDmut-do-for | CATAAATTCCCCGACATGGATTCCATAGGCGCAGCGATCGGGATTTTAAAG |
5623 | yybT-rev (BamHI) | GAAGGATCCTCATCTCTGTACGCCTCCCT |
5684 | yybS-int-up-for-(EcoRI) | GAGGAATTCTTGATATTGTAGAAACTGTAGCGA |
5685 | yybS-up-rev-(FLAG) | CATCCGCGGTTTATCATCATCATCTTTATAATCCATTTCTATCACTCCCCACCATGT |
5246 | yybT-for N-flag 1 | ATGGATTATAAAGATGATGATGATAAACCGCGGATGCCAAGCTTTTATGAAAAAC |
5686 | yybT-int-do-rev (BamHI) | GAGGGATCCATCCAATCCTTGTGTCACATCA |
5298 | yybT-for | AGTGATAGAAATGCCAAGCT |
Restriction sites are underlined.