FIGURE 4.

Quantitation of nitrated NO₂-Tyr¹⁶⁶-apoA-I present in human atherosclerotic lesions. A, recovery of apoA-I (μg) per gram of lesion tissue (wet weight) from the atherosclerotic lesion tissue homogenate and in the HDL-like and LPD fractions was calculated from quantitative Western blot analysis of apoA-I-immunoreactive bands, and total apoA-I content as described under “Experimental Procedures” is qualitatively presented in B. B, percentage of NO₂-Tyr¹⁶⁶-apoA-I to total apoA-I from atherosclerotic lesion present in the starting homogenate and HDL-like and LPD fractions was calculated as in A. C, percentage of NO₂-Tyr¹⁶⁶-apoA-I to total apoA-I within each fraction was determined by quantitative Western blot analysis of apoA-I-immunoreactive bands. Values were determined from n = 5 samples; error bars represent ±S.D.