FIGURE 5.
Myeloid Klf6 deficiency reduce cutaneous inflammation in vivo. A, Lyz2cre and Lyz2cre:Klf6fl/fl mice were subjected to TPA-induced cutaneous inflammation model. Ear weight from control and experimental groups was documented utilizing an analytical balance. The percentage increase in ear weight compared with the control is indicated. B, total protein extracts of ear tissue from vehicle and TPA-treated groups from Lyz2cre and Lyz2cre:Klf6fl/fl mice were subjected to myeloperoxidase activity assay. The data are indicated as MPO activity units/micrograms of total protein. C–I, total RNA from Lyz2cre and Lyz2cre:Klf6fl/fl mouse ear tissue exposed to TPA or vehicle control was analyzed for IL-1β, IL-6, MCP-1, TNF-α, MRC1, Arg1, and Retnla expression by quantitative PCR analysis. 36B4 was used as the housekeeping gene. The combined data from three experiments are shown. Data represent mean ± S.D., and a p value less than 0.05 between the indicated groups is considered significant.