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. 2014 Feb 18;289(15):10330–10344. doi: 10.1074/jbc.M113.546382

FIGURE 2.

FIGURE 2.

LIPUS suppresses adipogenic differentiation of 3T3-L1 cells. A, 3T3-L1 cells were induced to differentiate by a combination of dexamethasone, insulin, and IBMX for 12 days with or without daily stimulation by LIPUS for 20 min. Cells were stained with oil red O to determine lipid droplet appearance. B, 3T3-L1 cells were induced to differentiate by a combination of dexamethasone, insulin, and IBMX for 12 days with or without daily stimulation by LIPUS for 20 min. Total RNAs were isolated and reverse transcribed. The gene expressions of adipogenic markers were analyzed by real time PCR. The same experiments were performed at least three times. Relative mRNA expression levels are compared with Rpl13a. Error bars represent S.D. Statistical significance was determined by Student's t test (*, p <0.05; **, p < 0.01).