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2-Oxygenation of N-arachidonoylserotonin by P450 2U1. A, minus P450 2U1. B, plus P450 2U1. The scales are identical, and the tR of 2-oxo-N-arachidonoylserotonin is marked with the double-headed red arrow. The m/z 479 peak in the blank reaction (A) appeared at the position of N-arachidonoylserotonin and is attributed to a solvent adduct (+16), as revealed by fragmentation. The peak eluted at tR 16.8 min in B was not identified.
