FIGURE 5.

The DNA damage response is not altered in rad17Δ rtt106Δ cells. A, Rad52 recruitment during DNA damage was normal in rad17Δ rtt106Δ cells. Cells of the indicated genotype were left untreated or treated with CPT for 30 min. Rad52 foci, as determined from expression of Rad52 tagged with yellow fluorescent protein, Rad52-YFP, from S/G₂/M cells were counted. B, checkpoint activation is normal in mec3Δ rtt106Δ cells after Zeocin treatment. Cells of the indicated genotype were treated with Zeocin for the indicated amounts of time, and protein extraction was performed. Western blot was used to detect phosphorylation of H2AS129 (γ-H2AX). Sir2 and PCNA were used as loading controls.