TABLE 1.

A summary of genetic interactions among rtt106Δ and different checkpoint mutants

Cells containing mutations at the genes indicated in the left column, alone or in combination with rtt106Δ, were plated in a 10-fold serial dilution onto regular growth medium (YPD) or medium containing low concentrations of the indicated DNA damage agent (CPT, Zeocin HU, and MMS). Cell viability and growth were assessed over several days of incubation. A 0 represents no phenotypic growth effect over either single mutant (no synergistic effect). A + represents a synergistic defect, with additional +'s indicating a more severe growth phenotype. NA = not assessed. Sup = growth defects of single mutant were suppressed in double mutants.

Mutant	YPD	CPT	Zeocin	HU	MMS
rad17Δ	0	++	++	+	0
ddc1Δ	0	++	++	+	0
mec3Δ	0	++	++	+	0
mrc1Δ	0	0	0	0	0
Mrc1AQ	0	0	0	+	0
rad9Δ	0	+	+++	0	Sup
mec1–1	0	+++	NA	NA	NA
rad53–1	0	+	NA	NA	NA
rad24Δ	0	+++	NA	NA	NA
mrc1Δ rad17Δ	0	0	0	0	0
Mrc1AQ rad17Δ	0	0	0	0	0