TABLE 2.

Summary of the genetic interactions observed among CAC1, RTT106, and RAD17

The experiments were performed as described in Fig. 1, and representative images are shown in Fig. 2. Growth on regular growth medium (YPD) or medium containing a low concentration of the indicated DNA damage agent was assessed after 2–5 days of incubation. A 0 indicates no phenotypic difference between the mutant and wild type cells; a + represents a growth defect on the indicated media, with additional +'s indicating a more severe growth phenotype. Note that similar interactions were observed among CAC1 or RTT106 with DDC1 or MEC3, two other components of the 9-1-1 complex (Fig. 2 and data not shown).

Mutant	YPD	CPT	Zeocin	HU	MMS
rtt106Δ	0	+	+	0	0
cac1Δ	0	0	+	+	+
cac1Δ rtt106Δ	+	++	++	+	+
rad17Δ	0	+	0	++	+
rtt106Δ rad17Δ	0	++	++	+++	+
cac1Δ rad17Δ	0	++	++	++++	+++
cac1Δ rtt106Δ rad17Δ	+	+++	++++	+++++	+++