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. 2014 Feb 25;289(15):10530–10539. doi: 10.1074/jbc.M113.539270

FIGURE 7.

FIGURE 7.

Lipoprotein receptor activity of mLolB(His) derivatives with a mutation at Leu-68. A, the Pal(Strep)-LolA(FLAG) complex was purified as described under “Experimental Procedures” and incubated with wild-type mLolB(His) for 30 min at 4 °C or 30 °C. The reaction mixtures were then applied to TALON resin to adsorb mLolB(His). The resin bound (B) and unbound (U) fractions were analyzed by SDS-PAGE followed by immunoblotting with anti-LolB and -Pal antibodies. B, the Pal-accepting activity of the indicated mLolB(His) derivatives at 30 °C was examined as in A.