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. 2014 Feb 25;289(15):10530–10539. doi: 10.1074/jbc.M113.539270

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Membrane anchoring activity of mLolB(L68E). A, Pal(His)-mLolB (wt) and Pal(His)-mLolB(L68E) complexes were purified as described under “Experimental Procedures,” and aliquots (3 μg) were analyzed by SDS-PAGE followed staining with Coomassie Brilliant Blue R-250. The migration positions of molecular mass markers are shown on the left. B, the Pal(His)-mLolB (wt) and Pal(His)-mLolB(L68E) complexes were incubated with LolB-depleted outer membranes for 30 min at 30 °C. The reaction mixtures (input) were fractionated by ultracentrifugation into pellets (p) and supernatants (s) followed by analysis by SDS-PAGE and immunoblotting with anti-His tag antibodies.