FIGURE 5.

A SAP97-KSR1-GluA4 protein complex forms later in conditioning and interacts with protein kinases PKC and ERK. A–E, coimmunoprecipitation analysis of the timing of the protein-protein interactions induced by conditioning was evaluated in naïve, pseudoconditioned, and conditioned preparations that underwent training for the different time periods indicated. Except for the GluA4-stargazin (C) association that did not demonstrate conditioning-related changes, significant interactions were observed during conditioning beginning at C1 or C2. Input (naïve) and IgG lanes are also shown. *, significant differences from N. F, conditioning-dependent immunoprecipitation of KSR1 with p-ERK was inhibited by the MEK-ERK blocker PD98059 (50 μm) and stimulated by application of the PKA activator Sp-cAMPs (50 μm). *, significant differences from Ps2.