FIGURE 5.

Theoretical model of dimeric hTKFC with kinase substrates bound. The homodimeric model of hTKFC with bound DHA, ATP, and two Mg²⁺ ions per active site was constructed with Modeler using two Citrobacter sp. ATP-dependent DHA kinase structures as templates. The file describing the full model is available as supplemental File 3 (hTKFC_2DHA_2ATP.pdb). In the figure, the point of view and the orientation of the model are different in each panel. A, schematic representation of the complete model. B, detail of ATP bound in the L2-K1 (left) and K2-L1 (right) active sites. C, detail of amino acids near the Mg²⁺ ions and ATP.