FIGURE 3.

Ectopic expression of DOC2B suppresses growth and proliferation in SiHa and HeLa cells. A and B, the DOC2B ORF was cloned into retroviral vector pMX-IRES-GFP to generate pMX-DOC2B-IRES-GFP and used to transduce SiHa and HeLa cells. The expression of DOC2B upon ectopic expression was confirmed by both RT-PCR (A) and Western blot by anti-DOC2B antibody (B). β-Actin was used as internal control. C and D, representative image of the colony formation assay in SiHa and HeLa, respectively. E, representive quantitative analysis of the colony numbers in SiHa and HeLa cells, respectively. The ectopic expression of DOC2B significantly reduced the colony numbers in both SiHa and HeLa cells, respectively. F, ectopic expression of DOC2B inhibits SiHa and HeLa cell proliferation, respectively. G, representative colonies from SiHa and HeLa, respectively. Both colony number and size decreased upon DOC2B expression. H, DOC2B expression induced arrest at the G₀/G₁ and S phases of the cell cycle. *, p < 0.05 by independent Student's t test was considered as statistically significant. The experiments were repeated 3 times in duplicate.