TABLE 1.

List of primers used

The bold and underlined sequence represents the restriction sites incorporated for cloning.

Primer name	Sequence (5′-3′)	Product size	Annealing temperature
MS-DMSO-PCR
DOC2B-MS-DMSO-F	CCCGAGGTTAGAGTGCTGTG	1000 bp	64 °C
DOC2B-MS-DMSO-F	CTCCTGGATGCTGATGGTC
BGS
DOC2B-BGS-F	GTATGTGTGTATTTGTATATTTGYGTGTG	412 bp	61.8 °C
DOC2B-BGS-R	CCCCRAACRAC CCTAACTTAACCRCTACC
RT-PCR
DOC2B-RT-PCR-F	TGGTGTGGTTCTGGGCATCCACG	103 bp	60 °C
DOC2B-RT-PCR-R	TGGGAGCTCGCTGGTGAGCGTG
GAPDH-RT-PCR-F	GGCTCCCTTGGGTATATGGT	97 bp	60 °C
GAPDH-RT-PCR-R	TTGATTTTGGAGGGATCTCG
ACTB-RT-PCR-F	GACGACATGGAGAAAATCTG	132 bp	60 °C
ACTB-RT-PCR-R	ATGATCTGGGTCATCTTCTC
Promoter construct: luciferase essay
DOC2B-Promoter-F	TTGTGGGTACCCCCAGGGTCCCCTGATCAC	655 bp	65 °C
DOC2B-Promoter-R	CCGGAAAGCTTCGGCCCTGACTTGGCCGCT