. 2014 Feb 25;289(15):10637–10649. doi: 10.1074/jbc.M113.491506

TABLE 3.

Mean percentage methylation of DOC2B fragment in normal, tumor and cervical cancer cell lines

The following abbreviations are used: N1–N6, normal; T1–T18, tumor; N, negative; P, positive; LSIL, low grade squamous intra epithelial lesions; HSIL, high grade squamous intra epithelial lesions; PD-SCC, poorly differentiated squamous cell carcinoma; UD-SCC, undifferentiated squamous cell carcinoma; MD-SCC, moderately differentiated squamous cell carcinoma; LC-NK-SCC, non-keratinizing squamous cell carcinoma.

Samples	Tissue	Age	DOC2B methylation	HPV status
			%
N1	Normal	45	1.9	N
N2	Normal	50	7	N
N3	Normal	48	1.9	N
N4	Normal	42	1.9	N
N5	Normal	48	1.9	N
N6	Normal	55	1.7	N
Mean ± S.E.			2.72 ± 0.86^a
P1	LSIL	40	25	N
P2	LSIL	46	42.85	HPV16
P3	LSIL	46	26.92	HPV16
P4	LSIL	54	13.46	HPV18
P5	HSIL	53	19.23	HPV16
P6	HSIL	74	11.53	HPV11
Mean ± S.E.			23.17 ± 4.66^a
T1	PD-SCC (III-b)	68	19.23	N
T2	UD-SCC (II-b)	71	30.76	HPV18
T3	LC-K-SCC (III-B)	55	61.53	HPV16
T4	MD-SCC (II-b)	52	19.23	HPV16
T5	LC-NK-SCC (I-b)	40	1.9	HPV16
T6	LC-NK-SCC (I-a	57	24.48	HPV16
T7	LC-NK-SCC (I-a)	48	0	N
T8	UD-SCC (II-b)	55	17.3	N
T9	PD-SCC (II-a)	50	31.91	HPV16
T10	MD-SCC (III-b)	40	30.76	HPV18
T11	MD-SCC (III-b)	60	25	HPV16
T12	LC-NK-SCC (II-b)	49	86.66	HPV16
Mean ± S.E.			29.06 ± 6.93^a
SiHa			91.1	HPV16
CaSki			100	HPV16
HeLa			100	HPV18

^a According to one-way analysis of variance, the percentage of methylation was found to be statistically significant between normal and malignant samples. It was also found to statistically significant between normal and pre malignant and normal and malignant when Kruskal-Wallis test was performed. p value less than p < 0.05 was considered as statistically significant.