FIGURE 3.

Transient expression of vinculin partially rescues Certhrax-induced cell detachment. HeLa cells were transfected with pEGFP, pEGFP-vinculin, or pEGFP-paxillin ± pCerADPr. 20 h HPT, cells were washed 4 times in Opti-MEM and color micrographs taken. Integrity of cell adherence was measured by counting the cells remaining attached to the growth surface after 4 washes. Adherent cells were scored from 10 random fields for each transfection. A one-way ANOVA was performed on all columns. **, p < 0.01.