TABLE 1.

Mass spectroscopic analysis of the 120-kDa protein ADP ribosylated by CerADPr

Biotin-ADP-ribosylated 120-kDa protein band was excised and subjected to LC-MS/MS analysis (University of Massachusetts-Worcester Proteomic Center). The 8 most prevalent protein scores from two separate experiments are shown, ordered by total number of unique spectra. Scaffold Viewer was used to visualize results.

Identified proteins Homo sapiens	Accession number (human)	Molecular mass	Score (unique spectra)
		kDa
Analysis 1
Vinculin GN = VCL PE = 1 SV = 4	VINC	124	253
ATP-citrate synthase GN = ACLY PE = 1 SV = 3	ACLY	121	236
Eukaryotic translation initiation factor 3 subunit B GN = EIF3B PE = 1 SV = 3	EIF3B	92	84
Kinesin-1 heavy chain GN = KIF5B PE = 1 SV = 1	KINH	110	73
Eukaryotic translation initiation factor 3 subunit A GN = EIF3A PE = 1 SV = 1	EIF3A	167	70
Integrin β-1 GN = ITGB1 PE = 1 SV = 2	ITB1	88	62
α-Actinin-4 GN = ACTN4 PE = 1 SV = 2	ACTN4	105	51
Integrin α-V GN = ITGAV PE = 1 SV = 2	ITAV	116	46
Analysis 2
Vinculin GN = VCL PE = 1 SV = 4	VINC	124	107
Myosin-9 GN = MYH9 PE = 1 SV = 4	MYH9	227	88
Desmoplakin GN = DSP PE = 1 SV = 3	DESP	332	82
Plectin GN = PLEC PE = 1 SV = 3	PLEC	532	73
Filamin-A GN = FLNA PE = 1 SV = 4	FLNA	281	49
Eukaryotic translation initiation factor 3 subunit A GN = EIF3A PE = 1 SV = 1	EIF3A	167	35
Myosin-Ib GN = MYO1B PE = 2 SV = 3	MYO1B	132	34
Ankycorbin GN = RAI14 PE = 1 SV = 2	RAI14	110	33