FIGURE 5.

Effect of 4-PBA on HSP70 protein abundance in TALH cells of Umod mutant mice. HSP70 protein immunopositivity was predominantly detected in collecting duct cells of the renal papilla. In the outer medulla, the HSP70 protein was predominantly detected in collecting duct cells, but (nearly) no expression of HSP70 could be observed in uromodulin-expressing TALH cells. There were no obvious differences in HSP70 immunostaining pattern and intensity between different Umod genotypes or treatment groups (placebo control group versus 4-PBA group). The chromogen DAB for HSP70 and Vector RED for UMOD were used; nuclear staining was done with hemalum. The age of the mice analyzed was 4 month.