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. Author manuscript; available in PMC: 2014 May 28.
Published in final edited form as: Leukemia. 2013 Aug 27;28(1):147–154. doi: 10.1038/leu.2013.251

Figure 4. Combinatorial effect of LBH589 and 5-Aza on PTPN6 re-expression/CpG2 demethylation and DLBCL cell survival.

Figure 4

(A–B) Ly3 cells were treated with various concentrations of LBH or 5-Aza as indicated for 72 hours and PTPN6 (A) and pSTAT3 (B) were quantified by western blotting. (C) CpG2 methylation analysis in Ly3 cells treated with 5-Aza and LBH at indicated concentrations for 72 hrs. (D) DHL2 cells were treated with combinations of LBH (range, 2.5 to 25nM) and Aza (range, 25 to 250nM) for 72 hours and survival assessed by Annexin/PI flow cytometry. Results are depicted for the most synergistic (CI=0.4) combination (LBH 25 nM+Aza 250 nM). Bars represent mean ± SD from 3 independent experiments.