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. Author manuscript; available in PMC: 2014 May 28.
Published in final edited form as: Leukemia. 2013 Aug 27;28(1):147–154. doi: 10.1038/leu.2013.251

Figure 5. PTPN6 promoter 2 histone methylation analysis.

Figure 5

(A–B) ChIP assay demonstrating enrichment of the H3K9me3 and H3K27me3 on PTPN6 promoter 2 in Ly3 DLBCL cells, CD19+ normal B cells and Raji cells. A representative experiment out of three independent experiments is shown. (C) Effect of DZNEP (1μM) on PTPN6 mRNA expression (relative to GAPDH) in Ly3 cells. (D) ChIP assay in Ly3 cells demonstrates the effect of DZNep on HeK27me3 mark associated with PTPN6 promoter 2. (E) Effect of LBH589 on H3K9Ac mark associated with PTPN6 promoter 2 in Ly3 cells. (F) Effect of LBH589 (10 nM) on PTPN6 mRNA (relative to GAPDH) expression by qRT-PCR in Ly3 cells.