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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Prostaglandins Leukot Essent Fatty Acids. 2014 Feb 26;90(5):145–149. doi: 10.1016/j.plefa.2014.02.004

Fig. 3.

Fig. 3

The level of Smad2 protein is not affected by PGE2. Serum-starved HMC were stimulated with PGE2 for 2h. The TCL were collected and endogenous Smad2 protein was pulled down with by the Smad2L antibody, raised against a peptide near the link region of Smad2 protein. The precipitated Smad2 protein and Smad2 protein in TCL were examined by the Smad2L and Smad2N antibodies, respectively. β-actin was used as a loading control.