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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Prostaglandins Leukot Essent Fatty Acids. 2014 Feb 26;90(5):145–149. doi: 10.1016/j.plefa.2014.02.004

Fig. 4.

Fig. 4

Smad2 protein post-translational modification by PGE2. A, HMC were treated with 1µM of PGE2 or bio-PGE2 for 2h at 37°C. NeutrAvidin pulldown was then performed as described in experimental procedures. Resin-bound proteins were analyzed by immunoblotting with the Smad2L antibody. B, Purified 2 µg of GST or GST-Smad2 protein was incubated with 1µM bio-PGE2 for 2h at room temperature and then precipitated by NeutrAvidin-agarose. Resin-bound proteins were analyzed by immunoblotting with GST antibody and the Smad2L antibody, respectively.