Table 2.
R1= | -H Amt | ||||||||||
/ ID # | 1 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
S31N | |||||||||||
% inh/ | 35 ± 2/ | 1 ± 1 | 10 ± 1 | 20 ± 2 | 32 ± 2 | 3 ± 1 | 8 ± 1 | 26 ± 3 | 25 ± 2 | 8 ± 1 | 15 ± 1 |
IC50 | 199.9 μM | ||||||||||
WT | |||||||||||
% inh/ | 91 ± 3/ | 77 ± 1 | 31 ± 2 | 13 ± 2 | 3 ± 2 | 7 ± 2 | 3 ± 2 | 4 ± 3 | 4 ± 2 | 6 ± 1 | 6 ± 1 |
IC50 | 16.1 μM | ||||||||||
R1= | |||||||||||
/ ID # | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 |
S31N | |||||||||||
% inh/ | 12 ± 1 | 21 ± 2 | 14 ± 1 | 8 ± 2 | 8 ± 2 | 42 ± 2/ | 31 ± 2 | 17 ± 2 | 31 ± 2 | 19 ± 2 | 8 ± 2 |
IC50 | 166 μM | ||||||||||
WT | |||||||||||
% inh/ | 2 ± 2 | 45 ± 2 | 49 ± 2 | 36 ± 2 | 77 ± 1 | 67 ± 2 | 43 ± 1 | 26 ± 2 | 2 ± 2 | 16 ± 1 | 73 ± 2 |
IC50 |
The activity of the inhibitors was measured using the TEVC technique with full length AM2 protein in the Xenopus oocytes membrane; the average of three measurements was reported (see Experimental Section in the Supporting Information).