FIGURE 3.

Forcible targeting of Rab5A, an early endosome-resident Rab, to mature melanosomes in melanocytes by the MST tag. Typical images of melanocytes expressing mStr-tagged Rab5A and MST-Rab5A (mStr fluorescence images and their corresponding bright-field images). The melanosomes in the merged images (right column) are pseudocolored green. The insets show magnified views of the boxed areas. Note that MST-Rab5A was clearly targeted to mature melanosomes without altering peripheral melanosome distribution (bottom row). Scale bars = 20 μm.