FIGURE 2.

Influence of resting and activated platelets on MSC migration to apoptotic cardiac myocytes and fibroblasts. Conditioned media derived from staurosporine-treated (A and C) or sodium azide-treated (B and D) cardiac myocytes (A and B) or cardiac fibroblasts (C and D) served as chemoattractants for MSC in a transwell migration assay. In certain experiments, MSC were preincubated with conditioned media derived from resting (untreated) or activated (5 μg/ml CRP, 30 min; 50 μm ADP, 30 min) platelets for 24 h prior to evaluating migration. 24-h incubation of the MSC with CRP (5 μg/ml) or ADP (50 μm) in the absence of platelets served as controls. The number of migrating MSC was determined after 12 h. Data are presented as mean ± S.E. (error bars) for n ≥ 3. Statistical significance for the comparison with untreated MSC is indicated (*, p < 0.02; **, p < 0.01).