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. 2014 Mar 10;289(16):11111–11121. doi: 10.1074/jbc.M114.548529

FIGURE 1.

FIGURE 1.

A, TF-ELMOD1–3 proteins purified from bacteria. Each of the human ELMOD proteins was expressed in bacteria as N-terminal TF fusion proteins and purified as described under “Experimental Procedures.” Proteins (1 μg each) were resolved in 11% acrylamide SDS gels and stained with Coomassie Blue. B, GST-ELMOD1–3 proteins and ELMOD2 after cleavage of the GST tag, purified from HEK293T cells. Proteins were expressed in HEK cells, purified, and one (ELMOD2) was cleaved with TEV, as described under “Experimental Procedures” prior to loading samples (1 μg each) onto SDS-PAGE gels and staining with Coomassie Blue.