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GAP activities of GST-ELMOD2 against different ARF family GTPases. GAP activities against each of five different GTPases within the ARF family were determined using fixed concentrations of substrates and varying the amount of GST-ELMOD2, as described under “Experimental Procedures” and in the legend to Fig. 2. Note that the specific activities were calculated from the lower concentrations that fell along the straight part of each curve.
