FIGURE 4.

Binding of anti-O-GlcNAc antibodies to Lec1 cells. Lec1 cells (4 × 10⁵) fixed with 4% paraformaldehyde (Fix) or fixed and permeabilized with 0.1% Triton X-100 (Perm) were incubated with 10 μg/ml 10D8 or 40 μg/ml other anti-O-GlcNAc antibodies including isotype control Abs followed by incubation with 10 μg/ml secondary Ab. Gray profiles, secondary Ab; short dashed line, isotype control for Fix; long dashed line, isotype control for Perm; bold solid line, anti-O-GlcNAc mAb for Fix; thin solid line, anti-O-GlcNAc mAb for Perm. Representative results are from two independent experiments.