FIGURE 5.

GlcNAc competes for cell surface binding of only some anti-O-GlcNAc mAbs. Lec1 cells (4 × 10⁵) fixed with 4% paraformaldehyde were incubated with 10 μg/ml 10D8 mAb or 40 μg/ml each of the other anti-O-GlcNAc mAbs (CTD110.6, RL2, #3, #10, and #14) in the presence of 5 mm GlcNAc (bold line) or 5 mm GalNAc (dotted line), or no sugar (thin line) on ice for 20 min followed by 10 μg/ml secondary Ab. Gray profiles, secondary Ab alone. Representative results are from four independent experiments.