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. 2014 Mar 11;289(16):11162–11174. doi: 10.1074/jbc.M113.531855

FIGURE 8.

FIGURE 8.

LC3-TVS are associated with the nondegradative endosomal compartment. A and B, HEK293 cells expressing GFP-LC3 were treated with CPPs for 4 h, fixed, and stained with anti-LAMP1 followed by a Cy3-labeled secondary antibody. Arrows indicate puncta positive for both markers (A). A boxed area in panel c is enlarged in panel d. Panels e and f demonstrate the tubulovesicular nature of the puncta. The percentages of LC3- or LAMP1-positive puncta that were also positive for the other marker were calculated (B). C and D, HEK293 cells expressing GFP-LC3 were loaded with DQ-BSA for 2 h and then treated with CPPs for 4 h. Arrows indicate puncta positive for both markers (C). The percentages of LC3- or DQ-BSA-positive puncta that were also positive for the other marker were calculated (D). E, HEK293 cells were loaded with DQ-BSA for 2 h and then treated with CPPs for 4 h, fixed, and stained with anti-LAMP2. The percentages of LAMP2- or DQ-BSA-positive puncta that were also positive for the other marker were calculated. Scale bars, 5 μm (A) and 10 μm (C). Error bars, S.E.