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. 2014 Mar 13;289(16):11194–11205. doi: 10.1074/jbc.M113.529172

FIGURE 1.

FIGURE 1.

The miR-200b∼200a∼429 minimal promoter and its activity in epithelial and mesenchymal cell lines. A, nucleotide sequence of the −321/+19 minimal proximal miR-200b∼200a∼429 promoter with predicted Sp1 and AP-2 transcription factor binding sites relative to the transcription start site (TSS) are indicated. The putative binding sites for Sp1, AP-2, and ZEB sites are underlined and are numbered in order of occurrence from the 5′-end. B, expression of the miR-200b∼200a∼429 primary transcript (Pri-200ba429), ZEB1, Sp1, and AP-2α in various epithelial and mesenchymal cell lines. C, activity of the −321/+19 miR-200b wild type and ZEB binding site mutant promoters in epithelial and mesenchymal cells. Data are expressed as relative luciferase activity ± S.E. from three independent experiments relative to the activity of the promoter-less reporter set to a value of 1.