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. 2014 Mar 13;289(16):11194–11205. doi: 10.1074/jbc.M113.529172

FIGURE 4.

FIGURE 4.

Reciprocal binding of Sp1 and ZEB1 to the miR-200b promoter. A, ChIP assays were performed in epithelial MCF-7 and mesenchymal MDA-MB-231 cells for binding of Sp1 and ZEB1 to the miR-200b∼200a∼429 promoter. PCR primers shown in supplemental Table S3 were used to amplify various regions in the vicinity of the TSS. Data are shown as mean % input DNA control ± S.E. of combined results from at least three experiments. Significant differences between antibody binding in MCF-7 and MDA-MB-231 cells are analyzed by 2-way ANOVA with Bonferroni post-tests, **, p < 0.01; ***, <0.001. B, enrichment of Sp1 and ZEB1 binding at two regions within the E-cadherin promoter in MCF-7 and MDA-MB-231 cells. Primers for the −372/−280 E-cadherin promoter region are from Ref. 70. Data are shown as mean % input DNA control ± S.E. of combined results from three experiments. Significant differences between indicated pairs are analyzed by unpaired two-tailed Student's t test, **, p < 0.01; ***, p < 0.001.